Dr. Smail Meziane
European Institute of Antioxidants, France
Title: Measurement of skin antioxidant activity by the PAOT-Skin® Technology. Relationship with blood and urinary biomarkers of skin oxidative stress.
Biography
Biography: Dr. Smail Meziane
Abstract
According to Jones, oxidative stress (OS) has been defined as an imbalance between reactive oxygen species or ROS (including free radical and non-free radical species) and antioxidants in favor of the formers, leading to a disruption of the redox signaling and/or molecular damage to lipids, proteins and DNA. A promising but always under explored way to evidence OS is to use skin as a matrix. Having the largest surface area in the body, skin is a major target for oxidative stress, as it is continually exposed to external aggressions. The present talk is devoted to the potential use of the PAOT-Skin® technology based on electrochemical application for evidencing OS in a non-invasive way in the skin matrix. Data related to robustness and validation of the method will be examined.
In a study performed on 30 healthily subjects, we were able to evidence a negative and significant correlation between the PAOT-skin score ® and blood lipid peroxides (r = -0.43, p = 0.020), copper (r = -0.42, p = 0.022) and the Cu / Zn ratio (r = -0.49, p = 0.006). Statistical analysis revealed that the PAOT-skin score ®was positively correlated with the gamma-tocopherol/alpha-tocopherol ratio (r = 0.43; p = 0.020).
Other data on urine samples also indicate a positive correlation between PAOT-skin score® and the polyphenols/creatinine ratio (r = 0.41; p = 0.017) and a negative correlation with the oxidized DNA/creatinine ratio (r = -0.48; p = 0.0092).